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| Registros recuperados : 41 | |
| 21. |  | VENTURIERI, G. A.; RONCHI-TELES, B.; FERRAZ, I. D. K.; LOURDE, M.; HAMADA, N. Cupuaçu: a espécie, sua cultura, usos, e processamento. Belém, PA: Clube do cupu, 1993. 108 p. il.| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental. |
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| 23. | | MARSARO JÚNIOR, A. L.; NASCIMENTO, D. B.; RONCHI-TELES, B.; ADAIME, R. Faunistic analysis of the species of Anastrepha Schiner (Diptera: Tephritidae) in three municipalities of the state of Roraima, Brazil. Brazilian Journal of Biology, São Carlos, v. 72, n. 4, p. 813-819, Nov. 2012.| Biblioteca(s): Embrapa Amapá; Embrapa Trigo. |
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| 24. | | COSTA, S. G. M.; QUERINO, R. B.; RONCHI-TELES, B.; PENTEADO-DIAS, A.M.M.; ZUCCHI, R. A. Parasitoid diversity (Hymenoptera: Braconidae and Figitidae) on frugivorous larvae (Diptera: Tephritidae and Lonchaeidae) at Adolpho Ducke Forest Reserve, Central Amazon Region, Manaus, Brazil. Braz. J. Biol., v. 69, n.2, p. 363-370, 2009| Biblioteca(s): Embrapa Roraima. |
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| 25. | | GRIFFEL, S. C. P.; MARSARO JUNIOR, A. L.; LOVATO, L.; RONCHI-TELES, B.; SILVA, R. A. da. Ocorrência de Anastrepha spp. (Diptera: Tephritidae) em pomar comercial de goiaba em Roraima. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: resumos. Viçosa: UFV, 2008. 1 CD-ROM. Resumo 930-2.| Biblioteca(s): Embrapa Amapá. |
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| 26. | | MARSARO JUNIOR, A. L.; LOVATO, L.; RONCHI-TELES, B.; SILVA, R. A. da; GRIFFEL, S. C. P. Levantamento de hospedeiros e parasitóides de Anastrepha spp. (Diptera: Tephritidae) no município de Boa Vista, Estado de Roraima. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: resumos. Viçosa, MG: UFV, 2008. 1 CD ROM. Resumo 937-1.| Biblioteca(s): Embrapa Amapá. |
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| 27. | | MARSARO JÚNIOR, A. L.; ADAIME, R.; RONCHI-TELES, B.; LIMA, C. R.; PEREIRA, P. R. V. da S. Anastrepha species (Diptera: Tephritidae), their hosts and parasitoids in the extreme north of Brazil. Biota Neotropica, v. 11, n. 4, p. 117-123, dez. 2011.| Biblioteca(s): Embrapa Amapá; Embrapa Trigo. |
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| 28. | | PEREIRA, J. D. B.; SILVA, R. A. da; RONCHI-TELES, B.; GARCIA, M. V. B.; LEMOS, W. de P. Conhecimento sobre moscas-das-frutas no Estado de Rondônia. In: SILVA, R. A. da; LEMOS, W. de P.; ZUCCHI, R. A. (Ed.). Moscas-das-frutas na Amazônia brasileira: diversidade, hospedeiros e inimigos naturais. Macapá: Embrapa Amapá, 2011. p. 273-278.| Biblioteca(s): Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental. |
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| 30. | | MARSARO JUNIOR, A. L.; DEUS, E. G.; RONCHI-TELES, B.; ADAIME, R.; SILVA JUNIOR, R. J. da. Species of Anastrepha (Diptera: Tephritidae) captured in a guava orchard (Psidium guajava L., Myrtaceae) in Boa Vista, Roraima, Brazil. Brazilian Journal Biology, v. 73, n. 4, 2013.| Biblioteca(s): Embrapa Roraima. |
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| 31. | | MARSARO JÚNIOR, A. L.; DEUS, E. G.; RONCHI-TELES, B.; SILVA, R. A. da; SILVA JUNIOR, R. J. da. Species of Anastrepha (Diptera: Tephritidae) captured in a guava orchard (Psidium guajava L., Myrtaceae) in Boa Vista, Roraima, Brazil. Brazilian Journal of Biology, São Carlos, v. 73, n. 4, p. 879-886, 2013.| Biblioteca(s): Embrapa Trigo. |
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| 32. | | PASSO, J. F.; ZUCCHI, R. A.; RONCHI-TELES, B.; BARR, N. B.; MCPHERON, B. A.; ARAÚJO, E.; ADAIME, R.; SILVA, J. G. Phylogenetic analysis of Anastrepha obliqua (Macquart) populations in Brazil. In: MEETING OF THE TEPHRITID WORKERS OF THE WESTERN HEMISPHERE, 9th., 2016. Buenos Aires. Book of Abstracts... [Buenos Aires: FAO: AIEA, 2016]. p. 202.| Biblioteca(s): Embrapa Amapá. |
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| 33. | | SANTANA, G. da S.; RONCHI-TELES, B.; SANTOS, C. M. dos; SOARES, M. A.; FIDELIS, E. G.; AMARO, G. C.; SILVA, R. S. da. Global potential distribution, climate dynamics, and essential climate variables for Anastrepha suspensa (Diptera: Tephritidae) using the CLIMEX model. Theoretical and Applied Climatology, 2023.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Roraima. |
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| 34. | | SILVA JUNIOR, R. J. da; HALFELD-VIEIRA, B. de A.; NECHET, K. de L.; MARSARO JÚNIOR, A. L.; ABREU, R. L. S. de; RONCHI-TELES, B. Infecção de frutos de cupuaçu, Theobroma grandiflorum pelo fungo lasiodiplodia theobromae não depende de injúria causada por xyleborus affinis (coleoptera: scolytidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. não paginado.| Biblioteca(s): Embrapa Roraima. |
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| 35. | | MARSARO JÚNIOR, A. L.; RONCHI-TELES, B.; PEREIRA, J. D. B.; LIMA, C. R.; SILVA JÚNIOR, R. J.; SILVA, R. A. Assosciação de Doryctobracon Areolatus (hymenoptera: Braconidae) com Anastrepha SSP. (diptera: Tephritidae) no município de Pacarima, Estado de Roraima, Brasil. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 11., 2009, Bento Gonçalves. Resumos...Bento Gonçalves, 2009.| Biblioteca(s): Embrapa Roraima. |
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| 36. | | MARSARO JUNIOR, A. L.; RONCHI-TELES, B.; PEREIRA, J. D. B.; LIMA, C. R.; SILVA JUNIOR, R. J. da; SILVA, R. A. da. Associação de Doryctobracon areolatus (Hymenoptera: Braconidae) Comanastrepha spp. (Diptera: Tephritidae) no município de Pacaraima, estado de Roraima, Brasil. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 11., 2009, Bento Gonçalves. Tecnologia e conservação ambiental: resumos. [Bento Gonçalves]: Sociedade Entomológica do Brasil: IRGA: Unisinos: Fiocruz, 2009.| Biblioteca(s): Embrapa Amapá. |
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| 37. | | MARSARO JUNIOR, A. L.; RONCHI-TELES, B.; BARBOSA, R. I.; SILVA JUNIOR, R. J. da; AGUIAR, R. M. de; SILVA, R. A. da. Conhecimento sobre moscas-das-frutas no Estado de Roraima. In: SILVA, R. A. da; LEMOS, W. de P.; ZUCCHI, R. A. (Ed.). Moscas-das-frutas na Amazônia brasileira: diversidade, hospedeiros e inimigos naturais. Macapá: Embrapa Amapá, 2011. p. 279-290.| Biblioteca(s): Embrapa Amapá. |
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| 38. | | MARSARO JÚNIOR, A. L.; RONCHI-TELES, B.; BARBOSA, R. I.; SILVA JUNIOR, R. J. da; AGUIAR, R. M. de; SILVA, R. A. da. Conhecimento sobre moscas-das-frutas no Estado de Roraima. In: SILVA, R. A. da; LEMOS, W. de P.; ZUCCHI, R. A. (Ed.). Moscas-das-frutas na Amazônia brasileira: diversidade, hospedeiros e inimigos naturais. Macapá: Embrapa Amapá, 2011. p. 279-290.| Biblioteca(s): Embrapa Roraima. |
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| 39. | | SILVA JUNIOR, R. J. da; MARSARO JÚNIOR, A. L.; ABREU, R. L. S. de; RONCHI-TELES, B.; OLIVEIRA, L O. e.; AGUIAR, R. M. de. Cupins associados a espécies fritíferas e florestais no Estado de Roraima. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. não paginado.| Biblioteca(s): Embrapa Roraima. |
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| 40. | | SILVA, J. G.; PASSOS, J. F.; BAIA, D.; ADAIME, R.; LIMA, K.; ZUCCHI, R. A.; RONCHI-TELES, B.; RUIZ-ARCE, R.; BARR, N. B.; MCPHERON, B. A.; ARAÚJO, E. Genetic diversity in Brazilian populations of Anastrepha obliqua (Macquart) based on mtDNA. In: MEETING OF THE TEPHRITID WORKERS OF THE WESTERN HEMISPHERE, 9th., 2016. Buenos Aires. Book of Abstracts... [Buenos Aires: FAO: AIEA, 2016]. p. 199.| Biblioteca(s): Embrapa Amapá. |
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| Registros recuperados : 41 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
19/12/2017 |
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Data da última atualização: |
21/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
REIS, A. N. dos; MIRANDA, M. dos S.; SILVA, L. K. X.; SILVA, A. O. A. da; SOUSA, J. S. de; MORAIS, E. de; GARCIA, A. R.; DOMINGUES, S. F. S.; SILVA, J. K. do R. da; RIBEIRO, H. F. L. |
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Afiliação: |
Adriana Novaes dos Reis, UFPA; Moysés dos Santos Miranda, UFPA; Lílian Kátia Ximenes Silva, UNAMA; Aluizio Otavio Almeida da Silva, UFPA; José Silva de Sousa, UFPA; Eziquiel de Morais, IFPA; ALEXANDRE ROSSETTO GARCIA, CPPSE; Sheyla Farhayldes Souza Domingues, UFPA; Joyce Kelly do Rosário da Silva, UFPA; Haroldo Francisco Lobato Ribeiro, UFRA. |
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Título: |
Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 38, n. 6, p. 3613-3628, 2017. |
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DOI: |
10.5433/1679-0359.2017v38n6p3613 |
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Idioma: |
Inglês |
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Conteúdo: |
For artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. MenosFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evalu... Mostrar Tudo |
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Palavras-Chave: |
Powdered Coconut Water. |
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Thesaurus NAL: |
cryopreservation; Lippia. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169304/1/REIS-et-al-2016-Comparative-Evaluation-Between-TES-TRIS-and-ACP-112.pdf
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Marc: |
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100 1 $aREIS, A. N. dos
245 $aComparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm.$h[electronic resource]
260 $c2017
520 $aFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil.
650 $acryopreservation
650 $aLippia
653 $aPowdered Coconut Water
700 1 $aMIRANDA, M. dos S.
700 1 $aSILVA, L. K. X.
700 1 $aSILVA, A. O. A. da
700 1 $aSOUSA, J. S. de
700 1 $aMORAIS, E. de
700 1 $aGARCIA, A. R.
700 1 $aDOMINGUES, S. F. S.
700 1 $aSILVA, J. K. do R. da
700 1 $aRIBEIRO, H. F. L.
773 $tSemina: Ciências Agrárias, Londrina$gv. 38, n. 6, p. 3613-3628, 2017.
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Embrapa Pecuária Sudeste (CPPSE) |
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